4Z9N
ABC transporter / periplasmic binding protein from Brucella ovis with glutathione bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.990, 76.930, 131.470 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 31.655 - 1.745 |
| R-factor | 0.1524 |
| Rwork | 0.152 |
| R-free | 0.18280 |
| Structure solution method | SAD |
| Starting model (for MR) | phased from iodided soak |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.745 | 1.745 |
| Rmerge | 0.534 | |
| Number of reflections | 187580 | |
| <I/σ(I)> | 14.4 | 2.8 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | MCSG1b2: 25% PEG3350, 0.2M NaCl, 0.1M BisTris pH 5.5 |
