4Z9I
Asp-TarS from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2014-06-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.460, 59.510, 79.380 |
| Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
| Resolution | 42.000 - 1.570 |
| R-factor | 0.16793 |
| Rwork | 0.166 |
| R-free | 0.19849 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2asr |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.313 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.058 | |
| Number of reflections | 52820 | |
| <I/σ(I)> | 18.2 | 6.3 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 5.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | NaCl |
