4Z9E

Alba from Thermoplasma volcanium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97953
Spacegroup name	P 21 21 21
Unit cell lengths	80.263, 84.248, 84.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.490
R-factor	0.21371
Rwork	0.213
R-free	0.23286
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nfj
RMSD bond length	0.010
RMSD bond angle	1.453
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.490
Number of reflections	20513
<I/σ(I)>	43.8
Completeness [%]	99.7
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	HEPES, PEG 3350