4Z9D
EcPltA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.520, 55.660, 73.060 |
| Unit cell angles | 90.06, 104.07, 96.17 |
Refinement procedure
| Resolution | 35.894 - 1.800 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z9c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.179 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.800 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.300 |
| Number of reflections | 55479 | |
| <I/σ(I)> | 7.7 | 2.6 |
| Completeness [%] | 88.5 | 87.7 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Treated with 20 mM NAD+ for thirty minutes prior to crystallization, 1:1 protein to 4-7% PEG, 0.1 M Tris, pH 8.5, 0.15 M lithium sulfate |
