4Z9C
EcPltAB Oxidized
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 65 |
| Unit cell lengths | 160.900, 160.900, 62.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.312 - 2.350 |
| R-factor | 0.1951 |
| Rwork | 0.192 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dwa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.372 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.420 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.041 | 0.356 |
| Number of reflections | 38825 | |
| <I/σ(I)> | 12.1 | 2.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.4 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, 15% w/v PEG3350 |
