4Z98

Crystal Structure of Hen Egg White Lysozyme using Serial X-ray Diffraction Data Collection

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	293
Detector technology	PIXEL
Collection date	2014-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03318
Spacegroup name	P 43 21 2
Unit cell lengths	79.144, 79.144, 38.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.981 - 1.550
R-factor	0.2089
Rwork	0.205
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vdp
RMSD bond length	0.008
RMSD bond angle	1.078
Data reduction software	cctbx.xfel
Phasing software	PHASER
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.590	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	17566
<I/σ(I)>	14.58	3.68
Completeness [%]	96.9	91.6
Redundancy	7.9	3.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	3.5	277	20% w/v NaCl, 8% w/v PEG 8000, 0.5 M Sodium acetate