4Z98
Crystal Structure of Hen Egg White Lysozyme using Serial X-ray Diffraction Data Collection
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.144, 79.144, 38.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.981 - 1.550 |
| R-factor | 0.2089 |
| Rwork | 0.205 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vdp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.078 |
| Data reduction software | cctbx.xfel |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.590 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 17566 | |
| <I/σ(I)> | 14.58 | 3.68 |
| Completeness [%] | 96.9 | 91.6 |
| Redundancy | 7.9 | 3.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 3.5 | 277 | 20% w/v NaCl, 8% w/v PEG 8000, 0.5 M Sodium acetate |
