4Z97

Crystal structure of USP7 in complex with DNMT1(K1115Q)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 32 2 1
Unit cell lengths	123.659, 123.659, 152.928
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.861 - 2.998
R-factor	0.2121
Rwork	0.209
R-free	0.25430
RMSD bond length	0.008
RMSD bond angle	1.154
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.861	3.110
High resolution limit [Å]	2.998	3.000
Rmerge	0.072	0.744
Number of reflections	27615
<I/σ(I)>	22.6	1.8
Completeness [%]	99.8	99.9
Redundancy	5.1	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	297	0.1 M sodium citrate, pH5.5, 10% PEG6000, 15% glycerol