4Z92
crystal structure of parechovirus-1 virion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 399.500, 399.500, 332.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.000 - 3.100 |
| Rwork | 0.290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bev |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 3.150 |
| High resolution limit [Å] | 3.090 | 3.100 |
| Rmerge | 0.354 | 0.904 |
| Number of reflections | 233083 | |
| <I/σ(I)> | 5 | 0.9 |
| Completeness [%] | 78.2 | 31.8 |
| Redundancy | 5.4 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris pH 8.0, 0.6M ammonium sulfate, 0.1M MgCl2, 5% (w/v) glycerol |
