4Z8X

Truncated FtsH from A. aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-03-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	I 2 2 2
Unit cell lengths	127.860, 188.480, 206.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.510 - 3.250
R-factor	0.2275
Rwork	0.226
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2di4 2ce7
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000	3.330
High resolution limit [Å]	3.250	3.250
Rmerge	0.045	1.250
Number of reflections	39332
<I/σ(I)>	17.6	1.4
Completeness [%]	99.3	96.4
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	2 M ammonium sulphate, 2 mM EDTA, pH 7