4Z8S
Structural studies on a non-toxic homologue of type II RIPs from Momordica charantia (bitter gourd)-Native-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.970, 134.210, 44.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.470 - 2.360 |
| R-factor | 0.16652 |
| Rwork | 0.165 |
| R-free | 0.20144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hwm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.617 |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.470 | 2.490 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.110 | |
| Number of reflections | 36839 | |
| <I/σ(I)> | 12.2 | 3.18 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1M HEPES, 20% w/v PEG 10000 |
