4Z8J
Crystal structure of the SNX27 PDZ domain bound to the C-terminal PTHR PDZ binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.88560 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.070, 48.625, 55.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.851 - 0.950 |
| R-factor | 0.1436 |
| Rwork | 0.144 |
| R-free | 0.14440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qdo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.278 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.700 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.102 | 1.700 |
| Number of reflections | 64036 | |
| <I/σ(I)> | 11.7 | 1.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.1 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.1 M Bis/Tris (pH 5.5) and 2 M ammonium sulfate |
