4Z8G

Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2 (TL1 ABS2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-06
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.468, 53.936, 75.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.908 - 2.100
R-factor	0.1937
Rwork	0.191
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pki
RMSD bond length	0.015
RMSD bond angle	1.663
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.908	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.058	0.300
Number of reflections	12725
<I/σ(I)>	18.9	2
Completeness [%]	95.5	73.1
Redundancy	7	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.5	35% MPD, 0.1 M MES/NaOH, pH 6.0, 200 mM lithium sulfate