4Z8E
TEAD DBD mutant -deltaL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.590, 96.547, 84.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.830 - 2.092 |
| R-factor | 0.2246 |
| Rwork | 0.224 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.273 | 2.128 |
| High resolution limit [Å] | 2.092 | 2.092 |
| Rmerge | 0.058 | 0.752 |
| Number of reflections | 13625 | |
| <I/σ(I)> | 26.327 | 3.15 |
| Completeness [%] | 98.2 | 96.02 |
| Redundancy | 7.42 | 7.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 7-10 mg/mL protein, ammonium sulfate, MES/Tris-HCl, dioxane/PEG5000 MME |
