4Z88
SH3-II of Drosophila Rim-binding protein with Aplip1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-19 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.280, 62.420, 163.640 |
Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
Resolution | 45.250 - 2.090 |
R-factor | 0.21005 |
Rwork | 0.209 |
R-free | 0.23642 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2csq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.224 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.100 | 0.400 |
Number of reflections | 64269 | |
<I/σ(I)> | 7.7 | 2.6 |
Completeness [%] | 98.9 | 92.4 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 100 mM Bicine, 2.2-2.6 M Ammoniumsulfate |