4Z81
Crystal structure of AMA4 DI-DII-EGF1 from Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-26 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.220, 202.590, 72.800 |
| Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
| Resolution | 49.094 - 2.050 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 4Z80 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.782 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.650 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.095 | 0.502 |
| Number of reflections | 66314 | |
| <I/σ(I)> | 6.6 | 2.1 |
| Completeness [%] | 95.4 | 91.3 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M Bis-Tris pH 6.0, 25% PEG3350 |
