4Z7L

Crystal structure of Cas6b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0009
Spacegroup name	P 64
Unit cell lengths	180.570, 180.570, 119.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.748 - 3.503
R-factor	0.246
Rwork	0.243
R-free	0.28980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.175
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2247: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Number of reflections	54571
<I/σ(I)>	10.5
Completeness [%]	100.0
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	20 mM magnesium acetate, 50 mM sodium cacodylate pH 5.5, and 1.7 M ammonium sulfate