4Z7E

Soluble binding domain of Lmo1422 ABC-transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97734
Spacegroup name	P 1 21 1
Unit cell lengths	45.960, 62.870, 97.020
Unit cell angles	90.00, 91.71, 90.00

Refinement procedure

Resolution	48.488 - 1.500
R-factor	0.155
Rwork	0.153
R-free	0.19820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sw5
RMSD bond length	0.010
RMSD bond angle	1.242
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.025	0.800
Number of reflections	166913
<I/σ(I)>	13
Completeness [%]	96.0	97
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	25% PEG1500, 0.1 M PCTP