4Z7C
Diphosphomevalonate decarboxylase from the Sulfolobus solfataricus, space group h32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-01-26 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 152.130, 152.130, 108.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.260 - 2.200 |
| R-factor | 0.21243 |
| Rwork | 0.211 |
| R-free | 0.23709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hke |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.374 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.300 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 23567 |
| <I/σ(I)> | 22.6 |
| Completeness [%] | 96.4 |
| Redundancy | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M HEPES, 0.8M sodium phosphate monobasic, 0.8M potassium phosphate monobasic, 15% glycerol |
