4Z79

Leiomodin-1 Actin-Binding Site 2 (ABS2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-26
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	49.019, 52.192, 75.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.176 - 1.540
R-factor	0.1572
Rwork	0.156
R-free	0.17660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pki
RMSD bond length	0.010
RMSD bond angle	1.293
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.200	1.640
High resolution limit [Å]	1.540	1.540
Rmerge	0.037	0.436
Number of reflections	29387
<I/σ(I)>	23.9	2.4
Completeness [%]	98.6	92.2
Redundancy	5.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.5	0.1M Hepes, 10% Propanol, 20% Peg 3350