4Z79
Leiomodin-1 Actin-Binding Site 2 (ABS2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-26 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.019, 52.192, 75.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.176 - 1.540 |
R-factor | 0.1572 |
Rwork | 0.156 |
R-free | 0.17660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pki |
RMSD bond length | 0.010 |
RMSD bond angle | 1.293 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.200 | 1.640 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.037 | 0.436 |
Number of reflections | 29387 | |
<I/σ(I)> | 23.9 | 2.4 |
Completeness [%] | 98.6 | 92.2 |
Redundancy | 5.2 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.5 | 0.1M Hepes, 10% Propanol, 20% Peg 3350 |