4Z6K
Alcohol dehydrogenase from the antarctic psychrophile Moraxella sp. TAE 123
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-06 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 136.573, 136.573, 210.832 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 118.340 - 1.900 |
| R-factor | 0.21158 |
| Rwork | 0.210 |
| R-free | 0.23294 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.942 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.340 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 171943 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 96.6 | 72.1 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | reservoir solution: 100 mM Bis-Tris, 2 M ammonium sulphate, 100 mM NaCl, 100 mM imidazole. hanging drop volume 8 microL of protein and reservoir solutions (3:1). |
