4Z6J

Crystal structure of apo intact hoefavidin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96
Spacegroup name	P 62 2 2
Unit cell lengths	82.010, 82.010, 130.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.970 - 2.400
R-factor	0.23515
Rwork	0.232
R-free	0.28945
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ew1
RMSD bond length	0.013
RMSD bond angle	1.657
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.041	0.227
Number of reflections	10687
<I/σ(I)>	38
Completeness [%]	99.6
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5-1.8 M Li2SO4 and 0.1 M HEPES (pH 7.5-7.7)