4Z65
Carboxypeptidase B with Sulphamoil Arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.380, 79.380, 100.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.250 |
| R-factor | 0.15977 |
| Rwork | 0.159 |
| R-free | 0.18004 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.318 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 88471 | |
| <I/σ(I)> | 3.5558 | 0.11 |
| Completeness [%] | 99.4 | 96.35 |
| Redundancy | 13.51 | 7.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 293 | PEG 8000, Na-cacodylate, ZnAc2 |
