4Z62

The plant peptide hormone free receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-01
Detector	BRUKER SMART 6500
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	90.033, 98.790, 227.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.378 - 2.900
R-factor	0.205
Rwork	0.202
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.449
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.900
Number of reflections	21402
<I/σ(I)>	18.5
Completeness [%]	93.6
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Tris 8.5, 2.0 M (NH4)2SO4