4Z61
The plant peptide hormone receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-09 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 486.185, 73.495, 67.333 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.750 |
| R-factor | 0.2 |
| Rwork | 0.196 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.000 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 54303 |
| <I/σ(I)> | 14.7 |
| Completeness [%] | 88.8 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M sodium citrate pH 5.5, 0.4M KCl, 30%(v/v) Pentaerythritol propoxylate (5/4 PO/OH) |
