4Z5X
Glycogen phosphorylase in complex with gallic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2013-12-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.04 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.486, 128.486, 116.634 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.850 - 2.100 |
R-factor | 0.16058 |
Rwork | 0.159 |
R-free | 0.18928 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.337 |
Data reduction software | MOSFLM |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.360 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 56323 | |
<I/σ(I)> | 15.4 | 5.1 |
Completeness [%] | 98.4 | 97.8 |
Redundancy | 6.7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.8 | 289 | 10 mM BES BUFFER |