4Z5P
Crystal structure of the LnmA cytochrome P450 hydroxylase from the leinamycin biosynthetic pathway of Streptomyces atroolivaceus S-140 at 1.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.759, 75.454, 93.858 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 46.360 - 1.900 |
| R-factor | 0.1922 |
| Rwork | 0.191 |
| R-free | 0.21670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z5q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.360 | 46.360 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.028 | 0.833 | |
| Rmeas | 0.108 | 0.033 | 0.991 |
| Rpim | 0.056 | 0.017 | 0.530 |
| Total number of observations | 210829 | 2400 | 10076 |
| Number of reflections | 57513 | ||
| <I/σ(I)> | 11.1 | 37.9 | 1.6 |
| Completeness [%] | 94.9 | 96 | 70.1 |
| Redundancy | 3.7 | 3.5 | 3.2 |
| CC(1/2) | 0.997 | 0.998 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.77 | 298 | 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.77, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP |
