4Z5H

HipB(S29A)-O2 20mer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-02-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 3 2
Unit cell lengths	127.714, 127.714, 127.714
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.154 - 2.100
R-factor	0.1879
Rwork	0.187
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4Z52
RMSD bond length	0.007
RMSD bond angle	1.259
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.053	0.023	0.914
Total number of observations	479223
Number of reflections	21371
<I/σ(I)>	15
Completeness [%]	100.0	100	100
Redundancy	22.4	20.7	21.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	1 M sodium citrate