4Z59

HipB-O4 20mer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-01-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 3 2
Unit cell lengths	127.370, 127.370, 127.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.032 - 2.300
R-factor	0.1555
Rwork	0.152
R-free	0.18190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4Z52
RMSD bond length	0.010
RMSD bond angle	1.562
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.075	0.025	0.755
Total number of observations	312178
Number of reflections	16312
<I/σ(I)>	11.5
Completeness [%]	100.0	100	100
Redundancy	19.1	19.3	16.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	1 M sodium citrate