4Z54

High Resolution Human Septin3 GTPase domain with alpha-zero helix in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	43.410, 44.550, 78.950
Unit cell angles	99.29, 100.77, 108.39

Refinement procedure

Resolution	41.077 - 1.830
R-factor	0.1712
Rwork	0.170
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.047
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.080	41.080	1.880
High resolution limit [Å]	1.830	8.180	1.830
Rmerge	0.078	0.045	0.426
Number of reflections	45613
<I/σ(I)>	5.4
Completeness [%]	96.5	95.9	94.3
Redundancy	2.2	2.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	100mM Magnesium Formate, 15% PEG 3350