4Z51
High Resolution Human Septin 3 GTPase domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.280, 74.270, 79.210 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 75.117 - 1.860 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sop |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.820 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.120 | 75.120 | 1.910 |
| High resolution limit [Å] | 1.860 | 8.320 | 1.860 |
| Rmerge | 0.064 | 0.028 | 0.680 |
| Number of reflections | 23470 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 98.9 | 95.4 | 99.7 |
| Redundancy | 3.6 | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 50 mM magnesium acetate, 10mM Sodium Acetate, 15% PEG8000 |
