4Z4Z
Crystal structure of GII.10 P domain in complex with 30mM B antigen (trisaccharide)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.984463 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.830, 78.293, 71.630 |
| Unit cell angles | 90.00, 103.35, 90.00 |
Refinement procedure
| Resolution | 42.860 - 1.801 |
| R-factor | 0.1652 |
| Rwork | 0.164 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3onu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.860 | 1.865 |
| High resolution limit [Å] | 1.800 | 1.801 |
| Rmerge | 0.069 | |
| Number of reflections | 65787 | |
| <I/σ(I)> | 11.57 | 2.14 |
| Completeness [%] | 98.9 | 95.13 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium nitrate, bis-tris propane, PEG3350 |
