4Z4V
Crystal structure of GII.10 P domain in complex with 19mM fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.984463 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.109, 78.823, 68.656 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 43.000 - 1.800 |
| R-factor | 0.1498 |
| Rwork | 0.148 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3onu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.288 |
| Data reduction software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | |
| Low resolution limit [Å] | 43.050 | 1.863 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Rmerge | 0.382 | |
| Number of reflections | 62752 | |
| <I/σ(I)> | 16.73 | 3.02 |
| Completeness [%] | 98.8 | 95.1 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium nitrate, bis-tris propane, PEG3350 |
