4Z4U
Crystal structure of GII.10 P domain in complex with 37.5mM fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.984463 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.317, 78.774, 68.512 |
| Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
| Resolution | 43.100 - 1.890 |
| R-factor | 0.1592 |
| Rwork | 0.157 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3onu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 1.953 |
| High resolution limit [Å] | 1.885 | 1.885 |
| Rmerge | 0.078 | 0.608 |
| Number of reflections | 54715 | |
| <I/σ(I)> | 11.56 | 1.81 |
| Completeness [%] | 98.9 | 95.03 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium nitrate, bis-tris propane, PEG3350 |
