4Z4T
Crystal structure of GII.10 P domain in complex with 75mM fucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-20 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.984463 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.749, 78.850, 68.642 |
Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
Resolution | 43.419 - 1.800 |
R-factor | 0.1491 |
Rwork | 0.148 |
R-free | 0.17370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3onu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.141 |
Data reduction software | XDS |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.420 | 1.863 |
High resolution limit [Å] | 1.798 | 1.798 |
Rmerge | 0.044 | 0.187 |
Number of reflections | 63508 | |
<I/σ(I)> | 18.25 | 5.97 |
Completeness [%] | 98.8 | 96.05 |
Redundancy | 3.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium nitrate, bis-tris propane, PEG3350 |