4Z4M

Crystal structure of GFP-TAX1BP1 UBZ2 domain fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	67.731, 88.773, 91.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.870 - 2.150
R-factor	0.23737
Rwork	0.234
R-free	0.29818
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.023
RMSD bond angle	2.042
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	63.870
High resolution limit [Å]	2.150
Number of reflections	30758
<I/σ(I)>	6.8
Completeness [%]	97.3
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	293	0.2 M Ammonium fluoride, 20% w/v Polyethylene glycol 3,350