4Z4I

Human Argonaute2 A481T Mutant Bound to t1-G Target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99999
Spacegroup name	P 1 21 1
Unit cell lengths	55.595, 116.624, 69.622
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	44.687 - 2.801
R-factor	0.1938
Rwork	0.192
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w5o
RMSD bond length	0.006
RMSD bond angle	0.879
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.690	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.131
Number of reflections	21701
<I/σ(I)>	8.7
Completeness [%]	99.2
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tris, Isopropanol, Phenol, Magnesium