4Z4F
Human Argonaute2 Bound to t1-DAP Target RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.859, 116.591, 70.375 |
Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
Resolution | 35.460 - 2.800 |
R-factor | 0.1863 |
Rwork | 0.184 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w5o |
RMSD bond length | 0.006 |
RMSD bond angle | 0.903 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.860 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.098 | |
Number of reflections | 21078 | |
<I/σ(I)> | 9.9 | |
Completeness [%] | 95.0 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Tris, Isopropanol, Phenol, Magnesium |