4Z4F

Human Argonaute2 Bound to t1-DAP Target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	55.859, 116.591, 70.375
Unit cell angles	90.00, 92.52, 90.00

Refinement procedure

Resolution	35.460 - 2.800
R-factor	0.1863
Rwork	0.184
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w5o
RMSD bond length	0.006
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.860	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.098
Number of reflections	21078
<I/σ(I)>	9.9
Completeness [%]	95.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tris, Isopropanol, Phenol, Magnesium