4Z4C
Human Argonaute2 Bound to t1-C Target RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.692, 116.561, 69.606 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 35.305 - 2.303 |
| R-factor | 0.1717 |
| Rwork | 0.169 |
| R-free | 0.21860 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.850 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 38614 | |
| <I/σ(I)> | 13.1 | 2.2 |
| Completeness [%] | 98.4 | 96.3 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Tris, Isopropanol, Phenol, Magnesium |
