4Z48
Crystal structure of a DUF1329 family protein (DESPIG_00262) from Desulfovibrio piger ATCC 29098 at 1.75 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.400, 56.340, 65.560 |
| Unit cell angles | 100.71, 97.04, 109.01 |
Refinement procedure
| Resolution | 27.549 - 1.750 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.24770 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.549 | 27.549 | 1.800 |
| High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
| Rmerge | 0.069 | 0.504 | |
| Rmeas | 0.087 | 0.080 | 0.583 |
| Rpim | 0.044 | 0.041 | 0.293 |
| Total number of observations | 209473 | 2250 | 15380 |
| Number of reflections | 53315 | ||
| <I/σ(I)> | 8.8 | 19.4 | 2.2 |
| Completeness [%] | 95.6 | 92.6 | 94.2 |
| Redundancy | 3.9 | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2000M Sodium Iodide, 20.0000% polyethylene glycol 3350 |
