4Z45
Structure of OBP3 from the currant-lettuce aphid Nasonovia ribisnigri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 87.250, 87.250, 95.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.160 - 2.020 |
| R-factor | 0.16576 |
| Rwork | 0.164 |
| R-free | 0.20743 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.116 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.160 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.140 | 0.660 |
| Number of reflections | 27953 | |
| <I/σ(I)> | 17.5 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG 6000, 0.1 M Sodium Citrate pH 5 10mM ZnCl2 |
