4Z3U

PRV nuclear egress complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979200
Spacegroup name	P 43 21 2
Unit cell lengths	125.456, 125.456, 109.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.907 - 2.706
R-factor	0.2171
Rwork	0.212
R-free	0.27250
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.787
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.907	2.802
High resolution limit [Å]	2.706	2.706
Rmerge	0.087	0.617
Number of reflections	24223
<I/σ(I)>	10.41	2.02
Completeness [%]	99.4	96
Redundancy	4.55	4.21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.3 M NaSCN, 18% PEG3350, 0.3 M NaCl