4Z3N
Crystal structure of the MATE transporter ClbM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 79.460, 79.460, 174.921 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.730 - 2.700 |
R-factor | 0.2114 |
Rwork | 0.206 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vvn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.157 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.730 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.104 | 0.554 |
Number of reflections | 17649 | |
<I/σ(I)> | 8.5 | 2.6 |
Completeness [%] | 96.6 | 89.4 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.8 | 293 | PEG 500 DME, 100 mM lithium sulfate, 400 mM sodium chloride, 100 mM sodium cacodylate |