4Z3C

Zinc finger region of human TET3 in complex with CpG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	27.616, 54.744, 40.228
Unit cell angles	90.00, 103.53, 90.00

Refinement procedure

Resolution	39.110 - 1.570
R-factor	0.2164
Rwork	0.215
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hp3
RMSD bond length	0.017
RMSD bond angle	1.667
Data scaling software	Aimless (0.5.7)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.110	39.110	1.600
High resolution limit [Å]	1.570	8.600	1.570
Rmerge	0.070	0.040	1.070
Rpim	0.042	0.029	0.665
Total number of observations	59473	295	2848
Number of reflections	16318
<I/σ(I)>	10.4	27	1.1
Completeness [%]	99.7	90.4	99.8
Redundancy	3.6	3	3.5
CC(1/2)	0.998	0.995	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	30% PEG-1500, 0.2 M sodium chloride, 0.1 M HEPES