4Z3B
Crystal structure of MnCO/apo-R52CFr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.733, 181.733, 181.733 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.710 - 1.420 |
| R-factor | 0.16682 |
| Rwork | 0.166 |
| R-free | 0.17650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.815 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.710 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.071 | |
| Number of reflections | 48815 | |
| <I/σ(I)> | 60.7 | 60.7 |
| Completeness [%] | 99.8 | 99.78 |
| Redundancy | 20.6 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1M ammonium sulfate, 20mM cadmium sulfite |
