4Z39
Structure of OBP3 from the vetch aphid Megoura viciae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.220, 41.390, 44.310 |
| Unit cell angles | 100.28, 101.74, 105.03 |
Refinement procedure
| Resolution | 38.760 - 1.300 |
| R-factor | 0.12649 |
| Rwork | 0.125 |
| R-free | 0.16115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | fragments |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.780 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.760 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.108 | 0.590 |
| Number of reflections | 52318 | |
| <I/σ(I)> | 9.6 | 1.9 |
| Completeness [%] | 90.1 | 81.7 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.3 M AmSO4, 30% (w/v) PEG 4000, 7.5% (v/v) Glycerol |
