4Z2Y
Crystal structure of methyltransferase CalO6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.007 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 126.828, 126.828, 105.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.400 |
| R-factor | 0.32502 |
| Rwork | 0.325 |
| R-free | 0.33338 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.444 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 11759 |
| <I/σ(I)> | 28 |
| Completeness [%] | 98.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 294 | Reservoir: 0.1 M NaCl, 0.1 M Bis-Tris (pH 5.8), and 1.10-1.25 M ammonium sulfate |
