4Z2X

Crystal structure of a RNA binding domain of a U2 small nuclear ribonucleoprotein auxiliary factor 2 (U2AF) from mouse at 2.15 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97879
Spacegroup name	P 21 21 2
Unit cell lengths	59.130, 90.260, 37.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.900 - 2.150
R-factor	0.211
Rwork	0.209
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v4m
RMSD bond length	0.009
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.900		2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.098	0.016	0.950
Rmeas	0.113	0.019	1.093
Total number of observations	43198
Number of reflections	11422	151	843
<I/σ(I)>	12.09	54.7	1.8
Completeness [%]	98.2	90.4	98.1
Redundancy	3.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M sodium thiocyanate, 20% polyethylene glycol 3350