4Z2U
Crystal Structure of 2-hydroxybiphenyl 3-monooxygenase R242Q from Pseudomonas azelaica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-20 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.56 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.140, 131.540, 79.000 |
| Unit cell angles | 90.00, 98.28, 90.00 |
Refinement procedure
| Resolution | 32.373 - 2.500 |
| R-factor | 0.2054 |
| Rwork | 0.204 |
| R-free | 0.23730 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.304 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.140 | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.048 | 0.191 |
| Number of reflections | 49676 | |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 92.5 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium citrate and 25% PEG3350 |
