4Z2O
High resolution crystal structure of short hoefavidin-hoef-peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 37.940, 37.940, 155.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.960 - 1.170 |
| R-factor | 0.14018 |
| Rwork | 0.139 |
| R-free | 0.16576 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z27 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.344 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.210 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.062 | 0.602 |
| Number of reflections | 39465 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M NaCl, 0.1 M Bis-Tris pH 6.2-7.0 and 1.1-1.5 M ammonium sulfate |
