4Z29
Crystal structure of the magnetobacterial protein MtxA C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.344, 88.954, 95.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.700 - 2.030 |
R-factor | 0.1869 |
Rwork | 0.185 |
R-free | 0.22700 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.802 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | ARP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.030 | 5.510 | 2.030 |
Rmerge | 0.143 | 0.085 | 0.659 |
Total number of observations | 266426 | ||
Number of reflections | 22793 | ||
<I/σ(I)> | 12.4 | ||
Completeness [%] | 99.2 | 99.8 | 91.3 |
Redundancy | 11.7 | 12 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | 0.2 M sodium malonate pH 7.0, 25% PEG 3350 |